PCR Enhancer
Amplification of DNA fragments by PCR often demands optimization of reaction conditions, which lead to the removal of undesirable unspecifities and to the increase of amount of PCR product. Optimization includes the concentration of Mg2+, concentration of polymerase, temperature of annealing of oligonucleotide primers and others. In some cases optimization can be reached by addition of additives (e.g. DMSO or formamide). Interesting effects have been obtained with tetramethyl ammonium (TMA) oxalate (Kovářová and Dráber, Nucleic Acid Res., 28: e70, 2000), which is available under the commercial name PCR Enhancer. This enhancer is can be added directly into PCR mixture.