Plain PP Master Mix
Plain PP Master Mix is similar to PPP Master Mixes (Cat. No. P124 - P126) but does not contain a dye. Therefore, the Plain PP master Mix is extraordinarily suitable for routine PCR analysis by automatic detectors (capillary chromatography, real-time PCR and others). The mix contains Taq DNA polymerase, deoxyribonucleotides, reaction buffer components and additives. Samples for PCR are prepared by simple mixing Plain PP Master Mix with target specific oligonucleotide primers, template DNA and water. For qPCR analysis it is necessary to add fluorescent DNA-binding dye (such as SYBR Green I) or TaqMan probes. For gel electrophoresis analysis it is recommended to add a loading buffer (Cat. No P048, P062, P064 or P066).
| Cat.no. | Name | Package | Price | |
|---|---|---|---|---|
| P201 | Plain PP Master Mix | 40 reactions | 440,00 Kč | |
| P202 | Plain PP Master Mix | 200 reactions | 1 745,00 Kč | |
| P203 | Plain PP Master Mix | 1000 reactions | 6 980,00 Kč | |
| P203xl | Plain PP Master Mix | 4x 1000 reactions | 20 940,00 Kč |
Product description
Plain PP Master Mix is similar to PPP Master Mixes (Cat. No. P124 - P126) but does not contain a dye. Therefore, the Plain PP master Mix is extraordinarily suitable for routine PCR analysis by automatic detectors (capillary chromatography, real-time PCR and others). The mix contains Taq DNA polymerase, deoxyribonucleotides, reaction buffer components and additives. Samples for PCR are prepared by simple mixing Plain PP Master Mix with target specific oligonucleotide primers, template DNA and water. For qPCR analysis it is necessary to add fluorescent DNA-binding dye (such as SYBR Green I) or TaqMan probes.
For gel electrophoresis analysis it is recommended to add a loading buffer (Cat. No P048, P062, P064 or P066).
Rapid samples preparation
- All components of the Plain PP Master Mix are 2x concentrated, which facilitates rapid preparation of the PCR samples. The samples are prepared by mixing an aliquot of Plain PP Master Mix with oligonucleotide primers, template DNA, H2O (included) and if required fluorescent dye (e.g SYBR Green I).
- Plain PP Master Mix is especially useful for routine analysis of large sample numbers. To 0.5 ml of the Master Mix in original tube are added primers, PCR H2O and if required DNA dye, and the Mix is stored at -20 ± 5°C. Immediately before PCR, the Mix is thawed, aliquoted and DNA templates are added.
Optimized reaction buffer
- Reaction buffer in the Plain PP Master Mix enhances specificity and efficiency of PCR; it contains MgCl2 at a concentration optimized for majority of PCR.
- Stabilizers present in the Mix allow its storage for short periods at 4°C ± 3°C (days).
Technical data
Components and packaging
- 1 tube with 0.5 ml Plain PP Master Mix (for 40 reactions, 25 µl each)
- 1 tube with 1.5 ml PCR H2O.
Composition
- 2x concentrated Plain PP Master Mix contains: 150 mMTris-HCl, pH 8.8 (at 25oC), 40 mM (NH4)2SO4, 0.02% Tween 20, 5 mM MgCl2, 400 µM dATP, 400 µM dCTP, 400 µM dGTP, 400 µM dTTP, 100 U/ml Taq DNA polymerase, stabilizers and additives.
Storage
- For short terms (days) at 4°C ± 3°C.
- For long terms at -20 ± 5°C. Material can be repeatedly defrosted.
Purity and quality control
- Purity of Taq DNA polymerase is verified by SDS PAGE, only one band of 94 kDa is observed in Coomassie blue stained gel. Material is free of nucleases.
- Each batch of Plain PP Master Mix is tested for amplification of a single copy gene in genomic DNA.