M-MLV Reverse Transcriptase
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an enzyme for cDNA synthesis from single-stranded RNA in the presence of oligonucleotide primer.
| Cat.no. | Name | Package | Price | |
|---|---|---|---|---|
| M023 | M-MLV Reverse Transcriptase | 2000 U | 1 650,00 Kč | |
| M024 | M-MLV Reverse Transcriptase | 5x 2000 U | 6 600,00 Kč |
Product description
Description
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an enzyme for cDNA synthesis from single-stranded RNA in the presence of oligonucleotide primer.
Technical data
Enzyme concentration
- M-MLV RT is supplied at a concentration 200 U/μl.
Components and packaging
- 1 tube with M-MLV RT; 2 000 U/10 µl.
- 1 tube with 5x concentrated M-MLV RT react buffer, 200 µl.
- 1 tube of 100 mM dithiothreitol (DTT), 100 µl.
Unit definition
- One unit of M-MLV RT is defined as the amount of the enzyme which incorporates 1 nmol of dTTP into acid precipitable material during 10 min at 37oC; poly(A)-oligo (dT)12-18 is used as a template.
Storage
- At temperature -20oC ± 5°C.
Purity
- The enzyme is free of nucleases and is suitable for performing cDNA synthesis.
Composition
- Storage buffer: 20 mM Tris-HCl (pH 7.5 at 22oC), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% Nonidet P-40, 50% (vol/vol) glycerol.
- 5x concentrated M-MLV RT buffer (1st-strand buffer): 250 mM Tris-HCl (pH 8.3 at 22oC), 375 mM KCl, 15 mM MgCl2, stabilizer.