Hot Start DNA polymerases

Hot-Start DNA polymerases

Combi Taq DNA polymerase

In this product, concentration of Taq DNA polymerase is reduced to 1 U/μl. Furthermore, it possesses anti-Taq monoclonal antibody allowing hot start of PCR in which enzymatic activity of Taq DNA polymerase is blocked until the first denaturation step. After denaturation (at 94^oC), the antibody is irreversibly inactivated and enzyme becomes active. This decreases production of nonspecific DNA fragments.

Aptamer-Taq DNA polymerase

This products contains DNA aptamer anti-Taq is used instead of anti-Taq monoclonal antibody. When compared to antibody the aptamer has several advantages:
(1) it is not irreversibly denatured at temperature upto 100^oC,
(2) has low molecular weight, is prepared synthetically, and has well defined composition,
(3) is not contamined with components of mammalian cells,
(4) is resistant to action of proteases and allows preparation of PCR mixes at room temperature, and
(5) is less expensive.

Aptamer-Taq DNA polymerase, 5 U/µl

In this product, concentration of Taq DNA polymerase is 5 U/μl.
This products contains DNA aptamer anti-Taq is used instead of anti-Taq monoclonal antibody. When compared to antibody the aptamer has several advantages:

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Combi Taq DNA polymerase

Combi Taq DNA polymerase is a modification of Taq DNA polymerase Unis (Cat. No. T037-T039). The enzyme is supplemented with monoclonal antibody anti-Taq, which allows "hot-start" PCR. Furthermore, the polymerase is present at lower concentration (1 U/µl), which simplifies pipetting of the enzyme.

Aptamer-Taq DNA polymerase

Aptamer-Taq DNA polymerase is a modification of Combi Taq DNA polymerase (Cat. No. C205-C207) in which DNA aptamer anti-Taq is used instead of anti-Taq monoclonal antibody. When compared to antibody the aptamer has several advantages: (1) it is not irreversibly denatured at temperature upto 100oC, (2) has low molecular weight, is prepared synthetically, and has well defined composition, (3) is not contamined with components of mammalian cells, (4) is resistant to action of proteases and allows preparation of PCR mixes at room temperature, and (5) is less expensive.

Aptamer-Taq DNA polymerase, 5 U/µl

Aptamer-Taq DNA polymerase, 5 U/µl is a modification of Combi Taq DNA polymerase (Cat. No. C205-C207) in which DNA aptamer anti-Taq is used instead of anti-Taq monoclonal antibody.