PCR Master Mixes

Introduction

PCR master mixes significantly simplify procedures for preparation of PCR samples and analysis of PCR products. They are ready-to-use solutions usually containing all components required for PCR except primers and template DNA. Advantages of Top-Bio PCR mixes can be summarized as follows:

1. They possess all components of PCR optimized for compatibility.
2. PCR can be set up without mixing small amounts of individual PCR components.
3. They include additives, which contribute to simplified storage, transport and PCR efficiency of the mixes.

All these attributes enhance reproducibility of the PCR tests. The products are especially useful for routine PCR diagnostics and genotyping.

PCR mixes produced by Top-Bio

The following 2x concentrated PCR Mixes differ in composition and usage:

PPP Master Mix

• Basic type of PCR Master Mix containing all components including MgCl₂, additives and inert dye, which simplifies gel loading.

PPP Master Mix without MgCl₂

• Modified PPP Master missing MgCl₂ and thus allows optimization of PCR by fine tuning of Mg²⁺ concentration. MgCl₂ (25 mM) is provided in separate vial.

Plain PP Master Mix

• A modified PPP Master Mix without a dye.

TP 2x Master Mix

• TP 2x Master Mix is dedicated for universal analysis of DNA samples using PCR. It is based on recent finding that addition of Trehalose or 1,2-Propanediol (abbriated TP) into reaction mixture is capable of susbstantialy increasing efficiency of PCR and enhance amplification of samples  which are otherwise difficult to amplify, including DNA from whole blood, GC rich amplicons and samples containing PCR inhibitors (Horáková a spol., BMC Biotechnology, 11:41, 2011; Free PMC article).