Hot Start PCR Master Mixes

Hot Start PCR Master Mixes

Aptamer Hot Start Master Mix - NEW 2014

• Aptamer Hot Start Master Mix contains DNA aptamer anti-Taq which binds reversibly to Taq DNA polymerase, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. After PCR the aptamer regains its inhibitory activity.

Combi PPP Master Mix

• A modified PPP master mix supplemented with anti-Taq monoclonal antibody, which inactivates enzymatic activity of Taq DNA polymerase. The enzyme is activated after denaturation of the antibody during denaturation phase of PCR cycle. This modification leads to "hot start PCR".

Plain Combi PP Master Mix

• A modified Combi PPP Master Mix without a dye.

LA Hot Start Master Mix - New 2014

• LA (Long and Accurate). The Mix contains unique blend of two thermostable DNA polymerases. One of them is Taq DNA polymerase, which is highly processive but lacks a 3’->5’ exonuclease proofreading activity, which accounts for relatively high error rate during DNA amplification. Second polymerase is less processive but possesses 3’->5’ exonuclease activity necessary for proofreading. The polymerases mix decreases error rate during synthesis of complementary DNA strands and preserves high speed of DNA synthesis. The repair capability allows amplification of complex genomic DNA fragments (up to 15 kbps in size) and less complex viral DNA (up to 40 kbps in size) and significantly decreases error rate when compared to Taq DNA polymerase.