Carrier-GLY

Product description

For precipitation of small amounts of RNA or DNA 

Description

Carrier-GLY is a highly purified polysaccharide, glycogen, derived from oysters. It is an inert carrier, free of host DNA, RNA, nickases, DNases and RNases and is preferred over tRNA, yeast RNA or sonicated DNA as a carrier for the precipitation of nucleic acids, because it is less likely to interfere  with downstream applications and is more efficient in RNA and DNA precipitation. Low amounts (picograms/ml) of RNA or DNA oligonucleotides as short as 8 base pairs can be recovered using Carrier-GLY at a final concentration 20 µg/ml.

Technical data

Components and packaging

• Carrier-GLY is supplied as a highly purified glycogen in deionized sterile ultrapure water (18 Mohm.cm) at a concentration of ~20 mg/ml.   
• Basic packaging contains 1 ml of Carrier-GLY in 2 ml plastic vials with screw cap.
• Carrier-GLY can also be obtained as a part of a set for RNA or DNA precipitations, containing besides Carrier-GLY also Carrier-iRNA and Carrier-ACRYL, 1 ml each. Comparison of various carriers for RNA or DNA precipitation and key references are shown in  Table 1. 

Storage and Stability

• At temperature -20°C ± 5°C. Material can be repeatedly defrosted. Unopened vial is stable until the expiration date printed on the label

Quality control

Each batch of Carrier-GLY is analyzed in several assays. For the assays, DNA or RNA is examined in the Carrier Assay Buffer (CAB): 10 mM Tris-HCl, 2 mM MgCl₂, 1 mM dithiothreitol, pH 7.5 at 37^oC.

• Nucleic acid precipitation assay. Economy DNA marker (2.5 ul; Cat. No. D071) is mixed with 0.2 ml 10 mM Tris, 1 mM EDTA buffer, 1 ul Carrier/GLY, 20 µl of 3 M sodium acetate, pH 5.2, and 0.6 ml of 96% Ethanol. After 30 min at -20 ± 5°C the mixture is centrifuged at 12,000 x g, analyzed by electrophoresis in agarose gel with ethidium bromide and observed under UV light. More than 90% of all components of the DNA marker is recovered in the precipitate.
• Nick activity assay. Plasmid pUC19 (1 µg) in 200 µl CAB is incubated with Carrier-GLY (50 µg) for 1 hours at 37°C, followed by electrophoresis in agarose gel with ethidium bromide. No nicking activity is observed. 
• Ribonuclease assay. RNA (1 µg) in CAB is incubated with Carrier-GLY (50 µg) in 200 ul CAB for 1 hours at 37°C, followed by electrophoresis in agarose gel with ethidium bromide. No changes in properties of RNA are observed under UV light.
• Absence of nucleic acids. Carrier-GLY (200 µg) is loaded on agarose gel with ethidium bromide. After electrophoresis, no bands are observed under UV light.